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֪RcY 1
(wĴ̼a(chn)һDŽӑB(ti)^)cm(ҲoB(ti)mֲıoɫ)ɻzQ
﹤̰֣քeǻ̡:(μg)l(f)ø
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|Yԭ(4)DNAYԭ(3)DNAY(wn)ԭ(3)
СˮƽR¿ɿֱС0.2ļYСļ֧ԭw
|ĻԪCHONSԪ;ĻԪCHONPPԪ;ѪtԪCHONFe~GصԪCHONMgԪCHON;V|Ԫص֬
ԭ˼cТٟoĤʢڟoȾɫwۃHкwܼڳɷľǢzѭҎ(gu)ɢރHеĿz׃ǻͻ׃ߟoĤϵy(tng)Ya^(q)Bm(x);to˺;wѣMПoȡĤĺò
|W(wng)Ĥϵy(tng)cĤBcӺĤB߀cwĤB|MۯBMbǻȼӹγɾĤС\ݔ?sh)ߠwMһӹͷ
ڵпwɔ_(ǵ)øԭȍuL(jng)^ĤԼYЃ|W(wng)ߠwͼĤ
Nк˻Ҫȏƽ֣|SCȷ䡣ֻŷֳɼzͼ|zɷNʽvδֻֻ˥ϡѴΔ(sh)ԽļԽL˥̓ͬõĽY
ֻČ|ǻxԱ_DˮƽɻɂȷǾa^(q){ص
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ӰøٷٶȵøضȡAȵʹø׃ԵǏAߜwøĻԲضӰ-ømض60ҡ
̵ĹøԃøDNABø(cI);̵ĹøwSøzø(@ԭ|wrm˝ȵҺCȝBoԭ|w)ȵø(:)
ATPǼֱԴ|ڼȺ٣cADPDܵ\ݔźͷտs(jng)ЙCϳɵ;ܵНBv;γATPǺúⷴ
D׃ɻSWܕrӹwˮKwoø(NADP+)هB(ti)~GaˮNADPHγɵăɂʽ
߹ʵķ1LÕrg(һ݆)2ӹe(ֲg)3߹Ч(ٶ)
B܄(ˮͪƾ)ͨ^ĤĔUɢȑQĦȣǰٷ֝
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Ҫ(j)ֲͬͬˮͬ(ji)MУɲÇιMMй(ji)sˮ
oֳ(Ă)¡gֲMB(yng)ֲָ̥g(yu)cDZHă(yu)ԠֲMB(yng)ă(yu)cǣȡ١B(yng)ڶֳʸߡԄӻУٷֳ;oֲ;a(chn)ˎʳƷӄɫϡx;˹N;Dֲ
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֪RcY 2
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(1)WfĄ(chung)ʼʩRǺʩ
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(3)WfĄ(chung)MҪxǣʾκ΄ֲɼɵ'Ķfֲ֮gһHPϵ֮gHPϵʾMҪrֵ
2.Nӵļ
(4)Ȼϵy(tng)ČӴУMϵy(tng)wNȺȺ䡢B(ti)ϵy(tng)Ȧ
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չ
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Ȼl£ԭĿz׃ֻлͻ׃;Ŀz׃лͻ׃ؽMȾɫw׃
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(7)ܪĴxͷֳ^ֻĻM
չ
ٲǼȲԭҲ
ڽMÿNĻλķNÓķNǺ
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(9)ĤҪ֬|͵|M߀
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ټڵijɷc|Y϶ɵĻ
ڳwSøzøȥֲ:
֪RcY 3
1zy߀ԭԇ0.1g/mlNaOHҺ0.05g/mlCuSO4ҺMɣc߀ԭǰl(f)ɴutɫʹÕrעF(xin)F(xin)á
2bMе֬KȾҺKȾҺǰߌ֬ȾSɫȾɼtɫ
3bMеĵ|psԇʹÕrȼNaOHҺ2~3CuSO4Һɫj
4oȾɫwȾɫõȾɫđϻtҺ
5^DNARNAڼеķֲüG_tɷNȾɫȾɫDNAȾɾGɫRNAȾɼtɫ
6ԭ|ӰĤҺĤԼ@ɌĤ֮gļ|
7ǾGȾҺnjһȾwĻȾʹоwʬF(xin){Gɫ
8ڷڵĺϳӹ\ݔͷ^Pļw|W(wng)ߠww
9γҪͨ^Óˮsϵķʽ
10ҺȴڼҺȕrֲ:l(f)|ڷxF(xin);ҺСڼҺȕrֲ:l(f)|ڷxďԭF(xin)
֪RcY 4
һ WгҊWԪؼã
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2FeѪtĽMɳɷȱȱFؚѪѪtеFeǶrFrFDzõֲвٵԪһȱ۵ĽMܵ
3Mg~GwĽMԪءܶøļֲȱVr~׳F(xin)~}ʧG
4BM۵Ȱl(f)ͻ۹ܵLȱֲF(xin)
5Iנټصijɷȱ׃СY˕طԼנ[
6KѪ⛺^͕rF(xin)ļԄӹ(ji)ɮʧ
7NNǘ~GATP|ͺıԪNֲwγɵĻﶼDz(wn)ĻˮģNֲwȿƄȱNr~~N~SNһNˮB(ti)ϵy(tng)IB(yng)һNWԪˮB(ti)ϵy(tng)^NcPϕɸIB(yng)ڵˮB(ti)ϵy(tng)еĸIB(yng)Q顰ˮAںB(ti)ϵy(tng)еĸIB(yng)Q顰ೱwȱNHȱٰᣬ͕Ӱ푵wLl(f)
8PPǘ֬ATPıԪءֲwȱPӰ푵DNAďƺRNADĶӰ푵ֲLl(f)P߀cֲúͺеf^ATPADPжᡣPҲˮB(ti)ϵy(tng)IB(yng)һNԪֲȱPr~׳F(xin)o~Gϼtɫt
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Wгõԇ
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2Ƕԇ{ɫҺǻϺˮԡF(xin)utɫǵĜy
3psԇɷ֣0.1g/ml NaOHҺ0.01g/ml CuSO4Һ÷yҺȼ2mlҺuм3~4Һuyдڵ|tʬF(xin)ɫ
4K÷ȡKw95%ľƾuڙzy֬Ɍ֬ȾSɫKȾɼtɫ
5bDNADNAˮȾ{ɫ
6GbDNADNAGأȾ{Gɫ
750%ľƾҺ֬bKȾҺȾɫ50%ľƾҺϴȥɫ
875%ľƾҺښ75%ľƾܝB뼚ʹ|׃@ȣƾĝBÓˮÜp@tʹ浰|ѸÓˮ̳ĤKƾ75ľƾҺgǰQˎᘾǰƤwÓԼCе
995%ľƾҺswe֔(sh)95%ľƾDNA
1015%}95%ľƾҺweϿڽx
11đҺ(Ȟ0.01g/ml0.02g/ml)ȾɫwɫɌȾɫwȾɫͨȾɫ3~5ҲôtȾɫ
1220%ĸK3%^3.5%ȻFڱ^^øFe3+ĴЧrĸKк^ø
133%ĿԵҺ3%Һ2%røҺ̽øۺǵÌ
14Һb۵Ĵڡ׃{
15ͪȡ~Gwеɫ
16Һɷ֣20ʯ2ݱͪ1ݱ϶Ҳ93̖ͣɫصČɫڞVϷx_
17裺ɫصȡķxĥGɫ~Ƭrʹĥ
18̼}ĥGɫ~ƬrкЙCֹĥr~GwеɫƉġ
190.3g/mLҺஔ30%Һֲ:Һĝȴ|ڷx
200.1g/mLęcҺcuѪϣѪ
21ȻcҺٿܽDNAȻcȞ2mol/L 0.015mol/LrDNAܽȻcȞ0.14 mol/LrDNAܽڝȞ0.9%r}ˮ
22ȵøٿÁֽ⵰|ڿڄ:B(yng)rֽMʹMɢ
23ˮأ˹TwԇȰl(f)ķNӻ磬ʹȾɫwMӱԭǿڷѵļNwγ
24Ȼ}Ӽڵͨԣڻ̵Dʹ̎ڸܑB(ti)
֪RcYСP£
αһ֪RcY02-19
Y03-16
tlj֪RcY12-11
tlj֪RcY12-12
γ֪RcYͨ13ƪ08-30
Z֪Rc֮ZR06-20
֪Rc֪Rc07-28
ˮGij֪Rc10-19
СWZijԊ~֪Rc09-10